LOCUS       40924_48638       12257 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BAC cloning vector P[acman]-CmR-F-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Venken KJ, He Y, Hoskins RA, Bellen HJ.
  TITLE     P[acman]: a BAC transgenic platform for targeted insertion of large 
            DNA fragments in D. melanogaster
  JOURNAL   Science 314 (5806), 1747-1751 (2006)
  PUBMED    17138868
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   Venken KJT., He Y, Hoskins RA, Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2006) Molecular and Human Genetics, Baylor College
            of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 12257)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2006"; volume: "314"; issue: "5806"; pages: "1747-1751"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2006) Molecular and Human Genetics, Baylor College of 
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(244..717)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(793..1761)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1764..2936)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(3262..3512)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3518..4270)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(4361..4580)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4659..5372
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6236..6338
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             6339..6995
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    complement(7150..7382)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     7389..7405
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7415..7433
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7477..7551
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(7570..7588)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7624..11639
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(8108..8179,8581..8854,8929..9583,9645..9960,
                     10164..10295,10366..10980)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    complement(11640..12225)
                     /label=P element 5' end
ORIGIN
        1 tcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac ggcctgggta
       61 accaggtatt ttgtccacat aaccgtgcgc aaaatgttgt ggataagcag gacacagcag
      121 caatccacag caggcataca accgcacacc gaggttactc cgttctacag gttacgacga
      181 catgtcaata cttgcccttg acaggcattg atggaatcgt agtctcacgc tgatagtctg
      241 atcgacaata caagtgggac cgtggtccca gaccgataat cagaccgaca acacgagtgg
      301 gatcgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
      361 aataatcaga ccgacgatac gagtgggacc gtggttccag actaataatc agaccgacga
      421 tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt gggaccatgg
      481 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccagt ctgattatca
      541 gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg
      601 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc
      661 tgattatcag accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt
      721 tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac
      781 gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat
      841 acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa
      901 tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga
      961 cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc
     1021 ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa
     1081 ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc
     1141 aacaactgat ttaggcaatt tggcggtgtt gatacagcgg gtaataatct tacgtgaaat
     1201 attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc
     1261 ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa
     1321 tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag
     1381 tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg
     1441 accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc
     1501 gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc
     1561 aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa
     1621 agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg
     1681 ggcagctggt gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg
     1741 aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa
     1801 atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag
     1861 cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca
     1921 tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc
     1981 tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca
     2041 tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac
     2101 tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg gaacaatcag cacatcagca
     2161 gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca
     2221 tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc
     2281 agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag
     2341 gaaggaataa tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc
     2401 ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc
     2461 catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt
     2521 agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca
     2581 cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc
     2641 gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca
     2701 acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg
     2761 atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt
     2821 attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt
     2881 tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt
     2941 taatcattgc tttgcgtttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa
     3001 tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag
     3061 ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac
     3121 cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt
     3181 acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc
     3241 ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta
     3301 acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg
     3361 ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg
     3421 ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga
     3481 accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg gaagtgatat
     3541 cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca atgtatgaga
     3601 ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag cggcggcgga
     3661 agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc cagtcgattt
     3721 tcagagagac gatgcctgag ccatccggct tacgatactg acacagggat tcgtataaac
     3781 gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc gtaaaccggt
     3841 tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg taaagccctc
     3901 tggatggact gtgcgcacgt ttgataaacc aaggaaaaga ttcatagcct ttttcatcgc
     3961 cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcgaaactc ttcaatgcct
     4021 gccgtatatc cttactggct tccgcagagg tcaatccgaa tatttcagca tatttagcaa
     4081 catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt ctgatctggt
     4141 caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc gcctcagtga
     4201 ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg ataaccgctg
     4261 tttccgccat gacagatcca tgtgaagtgt gacaagtttt tagattgtca cactaaataa
     4321 aaaagagtca ataagcaggg ataactttgt gaaaaaacag cttcttctga gggcaatttg
     4381 tcacagggtt aagggcaatt tgtcacagac aggactgtca tttgagggtg atttgtcaca
     4441 ctgaaagggc aatttgtcac aacaccttct ctagaaccag catggataaa ggcctacaag
     4501 gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa tatccccgtg
     4561 gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca gaatatataa
     4621 gtgctgttcc ctggtgcttc ctcgctcact cgaaattccc ctttccgacg ctcaccgggc
     4681 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     4741 cgtcgaagcc gtgtgcgaga caccgcggcc ggccgccggc gttgtggata cctcgcggaa
     4801 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     4861 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     4921 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     4981 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     5041 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     5101 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     5161 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     5221 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     5281 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     5341 ggccgcgaac ggcctcaccc caaaaatggc aggggatctc gagggcttcg ccctgtcgct
     5401 cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat
     5461 taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca ccgcacgaag
     5521 atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg
     5581 acagaacact acttcctata aacgctacac aggctcctga gattaataat gcggatctct
     5641 acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt
     5701 ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca
     5761 aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc
     5821 aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt
     5881 tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata
     5941 cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt
     6001 cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc
     6061 cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat
     6121 gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
     6181 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
     6241 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     6301 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     6361 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     6421 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     6481 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     6541 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     6601 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     6661 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     6721 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     6781 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     6841 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     6901 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     6961 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     7021 gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     7081 attcgatgat aagctgtcaa acatgagaat tggtcgagat ccgctagctc acgatgagaa
     7141 tggccagacc atgatgaaat aacataaggt ggtcccgtcg gcaagagaca tccacttaac
     7201 gtatgcttgc aataagtgcg agtgaaagga atagtattct gagtgtcgta ttgagtctga
     7261 gtgagacagc gatatgattg ttgattaacc cttagcatgt ccgtggggtt tgaattaact
     7321 cataatatta attagacgaa attattttta aagttttatt tttaataatt tgcgagtacg
     7381 caaccggtgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     7441 ggagctcgtt taaacctcga gcggtccgtt atcgatggcg cgccggcctt aatggccgcg
     7501 gccgcattta aatgtcgact taattaaggc cggccgcgat cgcgcccggg cggtacccag
     7561 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     7621 tccgaattct agtatgtatg taagttaata aaacccattt ttgcggaaag tagataaaaa
     7681 aaacattttt tttttttact gcactggata tcattgaact tatctgatca gttttaaatt
     7741 tacttcgatc caagggtatt tgatgtacca ggttctttcg attacctctc actcaaaatg
     7801 acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa tatcgccgtc
     7861 tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac gtagcgtcaa
     7921 tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc
     7981 catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt
     8041 tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat
     8101 cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc
     8161 tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct
     8221 ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa
     8281 tcccaaaccg caattgcaca aacaaatagt gacacgaaac agattattct ggtagctgtt
     8341 ctcgctatat aagacaattt ttgagatcat atcatgatca agacatctaa aggcattcat
     8401 tttcgactat attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag
     8461 atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgg ggtacttttt
     8521 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     8581 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     8641 tacggcacgc tccggccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     8701 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     8761 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     8821 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     8881 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     8941 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     9001 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     9061 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     9121 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caagccatgg
     9181 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     9241 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     9301 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggctcta accgatccgc
     9361 cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc cacagcgtcg
     9421 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     9481 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     9541 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     9601 attaagggta tctagtatta cataacatct caactcctat ccagcgtggg tgcccagtgt
     9661 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     9721 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat tagcaaagta
     9781 gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact ggagcagccg
     9841 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     9901 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     9961 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
    10021 tagattttag atagaattgc ctgattccac acccttctta gtttttttca atgagatgta
    10081 tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata ttaatgagat
    10141 gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc
    10201 aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc ttcctgacca
    10261 acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag aatacatttg
    10321 catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag
    10381 ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg
    10441 ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc cactggtctt cacggcgatt
    10501 gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg
    10561 gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc
    10621 tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc
    10681 ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca
    10741 tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc
    10801 gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag
    10861 acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc
    10921 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag
    10981 ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt
    11041 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
    11101 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
    11161 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
    11221 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
    11281 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
    11341 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
    11401 ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa
    11461 tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct cacagctata
    11521 tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga
    11581 ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatg
    11641 ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt
    11701 attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga
    11761 ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta
    11821 tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc
    11881 ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcatta aagtgggtat
    11941 cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct
    12001 taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt
    12061 tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt
    12121 ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg
    12181 taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta
    12241 gtccagacta gtggatc
//
